Amouzadeh Tabrizi, M., Shamsipur, M., Saber, R., Sarkar, S
Herein, a novel electrochemical bi-aptasensor using green synthesized reduced graphene-poly(amidoamine)/gold nanocomposite (rGO-PAMAM/Aunano) as supporting matrix for covalent immobilization of flavin adenine dinucleotide (FAD) and thionine (Th) on the first (W1) and second (W2) surfaces of dual working electrode screen printed electrode has proposed. FAD and Th probes were immobilized by glutaraldehyde linker based on a reaction between primary amines (-NH2) of FAD and Th with -NH2 of rGO-PAMAM/Aunano modified dual working electrode for simultaneous determination of cytochrome c (CYC) and vascular endothelial growth factor (VEGF165) tumor markers.