Emami, T., Madani, R., Rezayat, S.M., Golchinfar, F., Sarkar, S.
The Western blot technique is a useful method for protein detection, but detection of small molecules of proteins or peptides is difficult. Nanotechnology makes peptide detection possible by making a metal-coated membrane of immobilin-polyvinylidene difluoride membranes. In this research, we used this technique for the diagnosis of avian influenza. Some immunodominant sequences of the nonstructural 1 protein of the avian influenza virus were selected with which to perform this technique for detection of antibodies in poultry sera. Gold nanoparticles were used to coat the immobilin-polyvinylidene difluoride membrane. This metal was able to bind to the functional groups of amino acids, such as the sulfhydryl group and the amine group, but it kept small molecules on the metal membrane and avoided diffusing peptides and small protein molecules to the metal-coated membrane during Western blotting. The size of the nonstructural 1 peptide was less than 2 kDa.