انتشارات / RCSTIM
تاریخ: 2017/05/01
توسط: Professor Saeed Sarkar
منتشر شده در: Sensors and Actuators, B: Chemical, 243, pp. 823-830
فایل های پیوست:
URL منتشر شده: https://www.sciencedirect.com/science/article/pii/S0925400516320238

Amouzadeh Tabrizi, M., Shamsipur, M., Saber, R., Sarkar, S., Zolfaghari, N.

Abstract



Here in, a high sensitive sandwich-type electrochemical immunosensor for the determination of SKBR-3 breast cancer cell has been fabricated using of green synthesized reduced graphene oxide as a platform to the immobilization of primary Herceptin antibody (Anti-HCT). The various reduced graphene oxide-tetrasodium 1, 3, 6, 8-pyrenetetrasulfonic acid/metal hexacyanoferrates (rGO-TPA/MHCFnano) nanocomposites included rGO-TPA/FeHCF, rGO-TPA/CoHCF, rGO-TPA/NiHCF and rGO-TPA/CuHCF were used as electrochemical labels of secondary Herceptine antibody. The obtained result showed that the sensitivity of proposed sandwich-type electrochemical immunosensor for the determination of SKBR-3 breast cancer cells (30,000 cells mL−1) using rGO-TPA/FeHCFnano labeled secondary Herceptine antibody was higher than the other rGO-TPA/MHCFnano-labeled secondary Herceptine antibodies. The differential pulse voltammetry (DPV) technique was used for the determination of SKBR-3 breast cancer cell in the concentration range of 500–30,000 cells mL−1 with the limit of detection of 21 cells mL−1. The proposed sandwich-type electrochemical immunosensor exhibited high selectivity, liner range responsibility and good stability.


https://www.sciencedirect.com/science/article/abs/pii/S0925400516320238?via%3Dihub